Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free:

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site:, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Google Analytics

Targeted advertising cookies


The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at or by post at:

24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal CBGP Cirad IRD SupAgro Muse

Home page

Emmanuelle Jousselin



NEWS: We are looking for a phd candidate to work on the evolution of di-symbiotic systems in aphids (see bottom of the page)

My long-term research interests are in the evolution of interactions among species and their role in the diversification of life. I address these questions through the use of phylogenetic reconstructions and phylogenetic comparative methods.

I first started by working on one of the most fascinating model systems in the study of coevolutionnary processes, the fig/ fig wasp interactions. Upon being hired by the INRA, I initiated a research program on the evolutionary history of aphids (Hemiptera:Aphididae), a group of sap-sucking insects that encompass many agricultural pests. I  started a fruitful collaboration with A. Coeur d’acier, an international expert in aphid taxonomy. We investigated the central role of host-alternation in aphids diversification in the Brachycaudus genus (ANR funded project: 2005-2008), looked at the role of host plant specialization and asexuality in the diversification of a world-wide pest (Brachycaudus helichrysi) (INRA funded project: 2008-2012), weighed the relative role of ecological speciation and geographic isolation in the diversification of Cinara, one the most species rich aphid genus (ANR funded project: Phylospace 2009-2014). We  are now investigating the role of symbiont evolutionary dynamics in aphids long term evolution (MicroPhAn project-France génomique).

All these projects have direct implications for aphid taxonomy and the sampling expeditions we have conducted (in Europe, Central Asia, Eastern Asia, North-America) to obtain comprehensive phylogenies of the groups we are studying,  are continuously enriching the INRA National Collection and the background knowledge we have on these  insects which are both fascinating models in Evolutionary Biology and important agricultural pests.


jo 452

                                                              The team on a field trip in the US.

Ongoing projects

Evolution of dual symbiotic systems in aphids: We have  conducted high-throughput sequencing of 16s rRNA gene and showed that in Cinara species, Buchnera has a “flatmate”: it co-exists with another obligate symbiont that has been repeatedely replaced. We are currently conducting phylogenomic analyses to investigate: 1) the history of these symbiotic associations, 2) the rates and patterns of genome evolution in these bacterial symbionts, 3) the role of symbiont evolutionary dynamics in the diversification of this globally distributed aphid genus (MicroPhAn project-AAP grand projet de séquençage France génomique). (Looking for a phd Student see bottom of the page!)

Diversification in conifer-feeding aphids (Lachninae:Cinara): our phylogenetic reconstructions of the Cinara genus aim at deciphering how host-plant adaptation processes and the climatic and geological changes that occurred through the Cenozoic  have impacted the diversification of this genus.

Ecological differentiation in a cosmopolitan pest: our ongoing work on the leaf-curl plum aphid (Brachycaudus helichrysi) has unravelled the existence of two sibling species in this cosmopolitan pest, differing in their primary host plant association. We have also demonstrated the repeated evolution of long term asexual lineages in one of the sibling species: it encompasses several “super-clones” that have dispersed over continents and persisted over a decade. We are currently conducting research to unravel the biotic and abiotic factors favouring the emergence of these superclones, and investigating whether endosymbionts play a role in their differentiation.

Life cycle complexity in aphids: by reconstructing a comprehensive phylogeny of the Brachycaudus genus, we have demonstrated that complex life-cycles (host-alternation) can be evolutionarily labile. This supports the hypotheses that life-cycles in aphids are adaptive: they evolve in response to natural selection. Using phylogenetic species delimitation methods on both Buchnera and aphids, we also demonstrated fine-scale cospeciation between the partners of the interaction. This showed that the Buchnera genome could be reliably used to infer investigate phylogenetic relationships in aphids.

Current Lab Members

Alejandro Manzano-Marin (Post-doctoral associate)

Armelle Coeur d'acier (Aphid Systamatics)

Anne-Laure Clamens (Molecular Biology)

Past-lab members

Andrea Sanchez-Meseguer (Post-doctoral associate)

Marianne Annonier (Master student)

Pierre Arnal (Master student)

Megan Popkin (Master student)

Joséphine Piffaretti (Phd student: 2009-2012)

Elsa call (Master student)


see also

Manzano-Marín A, Coeur d'acier A, Clamens A-L, Orvain C, Cruaud C, Barbe V & Jousselin E. 2019. Serial horizontal transfer of vitamin-biosynthetic genes enables the establishment of new nutritional symbionts in aphids' di-symbiotic systems. BioRxiv: 556274. doi:10.1101/556274.

Manzano-Marín A., Coeur d'acier A., Clamens A.-L., Orvain C., Cruaud C., Barbe V. & Jousselin E. 2018. A freeloader? The highly eroded yet large genome of the Serratia symbiotica symbiont of Cinara strobi. Genome Biology and Evolution 10(9): 2178-2189 (doi:10.1093/gbe/evy173)

Berry V., Chevenet F., Doyon J.P. & Jousselin E. 2018. A geography-aware reconciliation method to investigate diversification patterns in host/parasite interactions. Molecular Ecology Resources 18(5): 1173-1184 (doi:10.1111/1755-0998.12897)

Guyomar C., Legeai F., Jousselin E., Mougel C., Lemaitre C. & Simon J.C. 2018. Multi-scale characterization of symbiont diversity in the pea aphid complex through metagenomic approaches. Microbiome 6(1): 181 (doi:10.1186/s40168-018-0562-9)

Meseguer A.S., Lobo J.M., Cornuault J., Beerling D., Ruhfel B.R., Davis C.C., Jousselin E. & Sanmartín I. 2018. Reconstructing deep-time palaeoclimate legacies in the clusioid Malpighiales unveils their role in the evolution and extinction of the boreotropical flora. Global Ecology and Biogeography 27(5): 616-628 (doi:10.1111/geb.12724)

Meseguer A.S., Manzano-Marín A., Coeur d'Acier A., Clamens A.L., Godefroid M. & Jousselin E. 2017. Buchnera has changed flatmate but the repeated replacement of co-obligate symbionts is not associated with the ecological expansions of their aphid hosts. Molecular Ecology, 26, 2363-2378.

Popkin M., Piffaretti J., Clamens A.-L., Qiao G.-X., Chen J., Vitalis R., Vanlerberghe-Masutti F., Gupta R.K., Lamaari M., Langella O., Coeur d'acier A. & Jousselin E. 2017. Large-scale phylogeographic study of the cosmopolitan aphid pest Brachycaudus helichrysi reveals host plant associated lineages that evolved in allopatry. Biological Journal of the Linnean Society, 120, 102-114.

Kergoat G.J., Meseguer A.S. & Jousselin E. 2017. Evolution of Plant-Insect Interactions: Insights From Macroevolutionary Approaches in Plants and Herbivorous Insects. In: Insect-Plant Interactions in a Crop Protection Perspective (eds. Sauvion N, Thiery D & Calatayud PA), pp. 25-53.

Chevenet F., Doyon JP, Scornavacca C., Jousselin  E et V. Berry. 2016. SylvX: a viewer for phylogenetic tree reconciliations.  Bioinformatics 32(4), 608-613.

E. Jousselin, A.-L. Clamens, M. Galan , M. Bernard, S. Maman, B. Gschloessl,  G. Duport., F. Calevro, A. S. Meseguer, A. Coeur d’acier. 2016. An assessment of 16S rRNA amplicon Illumina sequencing procedure to study the microbiome of a symbiont rich aphid genus. Molecular Ecology Ressources. 16 (3) 628-640.

Meseguer, A. S., A. Coeur d'acier, G. Genson, et E. Jousselin. 2015. Unravelling the historical biogeography and diversification dynamics of a highly diverse conifer-feeding aphid genus. J. of Biogeography: 42:1482-1492.

Castel, G., Razzauti Sanfeliu, M., Jousselin, E., Kergoat, G., Cosson, J.-F. 2014. Changes in Diversification Patterns and Signatures of Selection during the Evolution of Murinae-Associated Hantaviruses. Viruses, 6, 1112-1134.

 Coeur D'Acier, A., Cruaud, A., Artige, E., Genson, G., Clamens, A. L., Pierre, E., Hudaverdian, S., Simon, J.-C., Jousselin, E., Rasplus, J. Y. (2014). DNA barcoding and the Associated PhylAphidB@se Website for the identification of European Aphids (Insecta: Hemiptera: Aphididae). Plos One, 9 (6).

Hubert, F., Grimm, G. W., Jousselin, E., Berry, V., Franc, A., Kremer, A. (2014).. Multiple nuclear genes stabilize the phylogenetic backbone of the genus Quercus. Systematics and Biodiversity 12 (4), 405-423.

Fornuskova, A., Vinkler, M., Pagès, M., Galan, M., Jousselin, E., Cerqueira, F., Morand, S., Charbonnel, N., Bryja, J., Cosson, J.-F. (2013). Contrasted evolutionary histories of two Toll-like receptors (Tlr4 and Tlr7) in wild rodents (MURINAE). BMC Evolutionary Biology, 13.

Jousselin, E., Cruaud, A., Genson, G., Chevenet, F., Foottit, R. G., Coeur d'Acier, A. (2013). . Is ecological speciation a major trend in aphids? Insights from a molecular phylogeny of the conifer-feeding genus Cinara. Frontiers in Zoology, 10.

Jousselin, E., Coeur D'Acier, A., Vanlerberghe-Masutti, F., Duron, O. (2013). Evolution and diversity of Arsenophonus endosymbionts in aphids. Molecular Ecology, 22 (1), 260-270.

Laamari, M., Coeur d'Acier, A., Jousselin, E. (2013). New data on aphid fauna (Hemiptera, Aphididae) in Algeria. Zookeys, 319, 223-229.

Piffaretti, J., Clamens, A. L., Vanlerberghe-Masutti, F., Gupta, R. K., Call, E., Halbert, S., Jousselin, E. (2013). Regular or covert sex defines two lineages and worldwide superclones within the leaf-curl plum aphid (Brachycaudus helichrysi, Kaltenbach). Molecular Ecology, 22 (15), 3916 - 3932.

Piffaretti, J., Rossi, J.-P., Vanlerberghe-Masutti, F., Genson, G., Coeur D'Acier, A., Jousselin, E. (2013). Molecular identification and ecological characteristics of two cryptic lineages within a cosmopolitan aphid pest, Brachycaudus helichrysi (Hemiptera: Aphididae). App. Ent. and Zoology, 48 (2), 155-164.

Cruaud, A., Ronsted, N., Chantarasuwan, B., Chou, L. S., Clement, W. L., Couloux, A., Cousins, B., Genson, G., Harrison, R. D., Hanson, P. E., Hossaert-McKey, M., Jabbour-Zahab, R., Jousselin, E., Kerdelhue, C., Kjellberg, F., Lopez Vaamonde, C., Peebles, J., Peng, Y.-Q., Santinello Pereira, R. A., Schramm, T., Ubaidillah, R., van Noort, S., Weiblen, G., Yang, D.-R., Yokpinyanee, A., Libeskind-Hadas, R., Cook, J. C., Rasplus, J. Y., Savolainen, V. (2012). An extreme case of plant-insect codiversification: figs and fig-pollinating wasps . Systematic Biology, 61 (6), 1029-1047.

Piffaretti, J., Vanlerberghe-Masutti, F., Tayeh, A., Clamens, A. L., Coeur d'Acier, A., Jousselin, E. (2012). Molecular phylogeny reveals the existence of two sibling species in the aphid pest Brachycaudus helichrysi (Hemiptera: Aphididae). Zoologica Scripta, 41, 266-280. x

Bonhomme, V., Gounand, I., Alaux, C., Jousselin, E., Barthélémy, D., Gaume, L. (2011). . The plant-ant Camponotus schmitzi helps its carnivorous host-plant Nepenthes bicalcarata to catch its prey. J. of Tropical Ecology, 27, 15-24.

Bonhomme, V., Pelloux-Prayer, H.,Jousselin, E., Forterre, Y., Labat, J.-J., Gaume-Vial, L. (2011). . Slippery or sticky? Functional diversity in the trapping strategy of Nepenthes carnivorous plants. New Phytologist, 191 (2), 545-54.

Lombaert, E., Guillemaud, T., Thomas, C. E., Lawson Handley, L. J. ., Li, J., Wang, S., Pang, H., Goryacheva, I., Zakharov, I. A., Jousselin, E., Poland, R. L., Migeon, A., van Lenteren, J., De Clercq, P., Berkvens, N., Jones, W., Estoup, A. (2011). . Inferring the origin of populations introduced from a genetically structured native range by approximate Bayesian computation: case study of the invasive ladybird Harmonia axyridis. Molecular Ecology, 20 (22), 4654 - 4670.

Laamari M, Jousselin E, Coeur d'acier A: 2010. Assessment of aphid diversity (Hemiptera: Aphididae) in Algeria: a fourteen-year investigation. Faunistic Entomology, 62(2):73-87.

Jousselin, E., G. Genson, et A. Coeur d'Acier. 2010. Evolutionary lability of a complex life cycle in the aphid genus Brachycaudus. BMC Evolutionary Biology 10.

J. Peccoud, J-C Simon, C von Dohlen, A Coeur d’acier, M Plantegenest, F Vanlerberghe-Masutti et E -Jousselin 2010.Evolutionary history of aphid-plant associations and their role in aphid diversification. Comptes Rendus Biologies, V 333, issue 6-7, P 474-487

Jousselin, E., Y. Desdevises, et A. Coeur d'Acier. 2009. Fine-scale cospeciation between Brachycaudus and Buchnera aphidicola:  bacterial genome helps define species and evolutionary relationships in aphids. Proceedings of the Royal Society B 276:187-196.

Tayeh et al. dans Anderson CM, Aparicio GJ, Atangana AR, Beaulieu J, Bruford MW, Cain F, Campos T, Cariani A, Carvalho MA, Chen N et al: Permanent Genetic Resources added to Molecular Ecology Resources Database 1 December 2009-31 January 2010. Mol Ecol Resour 2010, 10(3):576-579. (Primer Note)

Jousselin, E. , S.  van Noort, V.  Berry, J.Y. Rasplus, N.  Ronsted, J.C.  Erasmus, et J..M.  Greeff 2008. One fig to bind them all: host conservatism in a Fig wasp community unraveled by cospeciation analyses among pollinating and nonpollinating fig wasps. Evolution 62:1777-1797.

Coeur d'Acier, A., G. Cocuzza, E. Jousselin, V. Cavalieri, et S. Barbagallo. 2008. Molecular phylogeny and systematic in the genus Brachycaudus (Homoptera: Aphididae): insights from a combined analysis of nuclear and mitochondrial genes. Zoologica Scripta 37 (2):175-193.

Cocuzza, G., E. Jousselin, V. Cavalieri, A. Coeur d'Acier, et S. Barbagallo. 2007. Morphological and Molecular analysis of Brachycaudus, subgenus Appelia-complex (Rhynchota, Aphididae). Redia 90:33-49.

Coeur d'Acier, A., E. Jousselin, J.F. Martin, & J.Y. Rasplus. 2007. Phylogeny of the genus Aphis Linnaeus, 1758 (Homoptera: Aphididae) inferred from mitochondrial DNA sequences. Molecular Phylogenetics and Evolution 42:598-611.

Erasmus, J.C., S. Van Noort, E. Jousselin, & J.M. Greeff. 2007. Molecular phylogeny of fig wasp pollinators (Agaonidae, Hymenoptera) of Ficus section Galoglychia. Zool. Scripta 36 (1):61-78

Jousselin E., van Noort S., Rasplus, J-Y, Greeff J.M.  2006. Patterns of diversification of Afrotropical Otiteselline fig wasps: phylogenetic study reveals a double radiation across host figs and conservatism of host association. Journal of Evolutionary Biology. . 19: 253-266.

Jousselin E., van Noort S., & Greeff J.M.  2004. Labile male morphology and intraspecific male polymorphism in the Philotrypesis fig wasps.  Molecular Phylogenetics and Evolution. 33: 706-718

Jousselin E. Kjellberg, F., Herre, E.A. 2004. Flower specialisation in a passively pollinated monoecious fig: a question of style and stigma? Int. J. of Plant Science. 16S (4): 587-593

Yu, DW J Ridley,  E Jousselin, EA Herre, SGA Compton, JM Cook,  JC Moore & GD Weiblen. 2004. Oviposition strategies, host coercion, and the stable exploitation of figs by wasps. - Proceedings of the Royal society of London.  271: 1175-1183

Jousselin E., Hossaert-McKey, M., Herre E.A. & Kjellberg F. 2003. Why actively pollinate monoecious figs ?- Oecologia 134: 381-387

Jousselin E., Rasplus J.Y & Kjellberg F. 2003. Convergence and coevolution in a mutualism: evidence from a molecular phylogeny of Ficus - Evolution 57: 1255-1269.

Jousselin E., Rasplus J.Y & Kjellberg F. 2001. Shift to mutualism in a parasitic lineage of the fig/fig wasp mutualism - Oikos : 2 : 287-294

Jousselin E., Hossaert-Mckey M., Vernet D. & Kjellberg F. 2001. Egg deposition pattern in fig wasps: implication for studies on the mutualism stability - Ecological entomology, 26:602-608

Jousselin E. et Kjellberg F. 2001. The functional implications of active and passive pollination in dioecious figs - Ecology letters  4 :151-158

Machado C., Jousselin E., Kjellberg, F., Compton S.G. et Herre E. A. 2001. Phylogenetic relationships, historical biogeography, and characters evolution of fig pollinating wasps – Proc. R.  soc . London B. 268 : 685-694

Kjellberg F., Jousselin E., Bronstein J., Patel A & Rasplus J.Y 2001. Pollination mode in fig wasps : the predictive power of correlated traits- Proc. of the Royal society of London B. 268 : 1113-1121

Book chapters

Nieberding, C., E Jousselin et Y. Desdevise. 2010. The use of .co-phylogeographic patterns to predict the nature of host-parasite interactions, and vice versa, dans “The biogeography of Host-Parasite Interactions” Edité par S. Morand, B.R. Krasnov.  p 60669

Ravigné V.(dir. Chap. 4), Barberousse A., Bierne N., Britton-Davidian J., Capy P., Desdevises Y., Giraud T., Jousselin E., Moulia C., Simadja C. & Samadi S. 2010. La spéciation. In: Thomas, F., Lefevre, T. & Raymond, M., ed. Biologie évolutive. de Boeck, Bruxelles & CNRS, pp. 79-122

Kjellberg F., Jousselin E., Hossaert-Mc Key M., Rasplus J.Y. 2005. Biology, Ecology and Evolution of Ficus (Moraceae) pollinating wasps (Chalcidoidea, Agaonidae). In Biology, Ecology and Evolution of Gall-inducing arthropods. Volume 2. Raman, A Schaefer C.W., Withers T.M. (eds) inc., Enfield, New Hampshire

Jousselin, E., J-F Sylvain et G.Kergoat . 2013. Les scénario évolutifs d’interactions entre insectes phytophages et plantes, ( Partie 7 - Chapitre 35). dans des « Insectes et des Plantes ». Edité par N. Sauvion, D. Thierry, P.A. Calatayud, F. Marion-Poll. IRD & Publibook

See also

We are Looking for a phd candidate to work on the evolution of di-symbiotic systems in aphids (start date no longer than 1st of October 2019, the Phd Grant is funded for three years)



 Background: Phytophagous insects are an important part of terrestrial biodiversity. A key factor in their evolutionary success probably lies in the mutualistic associations they have established with some intracellular bacteria. These endosymbionts metabolize some of the nutrients lacking in their host diet and can be essential for successfully shifting to new ecological niches. However long-term obligate associations with bacterial partners have their downsides. Endosymbiotic bacteria generally exhibit drastic genome reduction in comparison with their free-living relatives: this is due to relaxed evolutionary constraints on genes that are not necessary for life within the host and genetic drift caused by severe bottlenecks occurring during the maternal transfer of endosymbionts. This drift leads to the fixation of mildly deleterious mutations and can ultimately alter endosymbiont genomes and increase host vulnerability to environmental changes. This process of genome erosion can sometimes be compensated by the recruitment of a new symbiont that complements the primary one; such multi-partner endosymbioses have been observed in several insect lineages. The aim of this thesis is to study the evolutionary dynamics of a di-symbiotic system in aphids.

    Aphids have established an obligate mutualistic relationship more than 100 million years ago with a bacterium; Buchnera aphidicola, which compensates for their sugar-rich phloem diet by providing them with essential amino acids and vitamins. In some aphid lineages, this mutualistic relationship has evolved into a tri-partite association in which Buchnera aphidicola is "assisted" by a second bacterial partner that has been repeated replaced along the evolutionary history of the aphids (Manzano-Marín  2017, Meseguer et al. 2017). These shifts in symbiotic associations can be associated with the genome erosion of one the symbionts (Manzano-Marín et al. 2018) and favored by Horizontal Gene Transfers between symbionts (Manzano-Marín et al., 2019).


 Using Cinara aphids as a study system, the aim of the thesis will be to:

1) reconstruct the history of aphid/endosymbiont associations using phylogenomic analyzes and cospeciation analyzes ;

2) characterize patterns of molecular evolution in bacterial lineages and identify the signatures of selection and drift in bacterial genomes;

3) investigate  phenotypic and intra host dynamics  of obligate endosymbionts.


 We are looking for a student with a solid background in phylogenetics and/or evolutionary genomics and a strong interest in addressing evolutionary questions in host/symbiont associations. He will be trained in the comparative genomics of bacterial genomes and techniques for investigating symbiont dynamics within hosts. 


 Host laboratory and context:

The Phd will be supervised by E. Jousselin and A. Manzano- Marín at CBGP (Montpellier)

He/she will analyze an already acquired dataset (endosymbiont genome data from about 50 aphid species) using phylogenomics.

He /she will join a collaborative network :

-Bastien Boussaud and Vincent Daubin (Univ. Lyon, France) to implement host/symbiont reconciliation analyses and investigate patterns of molecular evolution in endosymbiont genomes.

-F. Calevro (Bf2i, INSA, Lyon) to investigate cell dynamics of aphid di-symbiotic systems.


For any enquiry contact


Expected start date : no later than 1st of October 2019

To apply, please send a pdf file with

(i) Cover letter with a brief summary of previous experience, academic background and motivation for the position

(ii) CV 

(iii) Names and contact details of 2 references.

Deadline for application: 24th of july

Informal enquires are welcome.

Salary: about 20 K€/year net, including health insurance