Because of the structural diversity, characterisation of phenolic compounds in plants and plant based food and non food products, requires an integrated analytical approach. The polyphenol platform thus offers analytical, methodological and technical help, as well as expertise in mass spectrometry, nuclear magnetic resonance and chemometrics for :
· structural characterisation
· quantification
· high throughput analysis of large series of samples
The Polyphenol platform is a labelled analytical facility since 2003: GIS IBiSA (Infrastructures en Biologie Santé et Agronomie), CNOC (INRA) and PRRI (Région Occitanie Pyrénées-Méditerranée).
Organisation
The Polyphenol platform hosts three divisions :
· NMR : Dr. Christine Le Guernévé
· Mass spectrometry : Dr. Nicolas Sommerer, Mrs. Emmanuelle Meudec and Mr. Arnaud Verbaere
· Chemometrics : Dr. Jean-Claude Boulet
Request for analysis
For each request, please fill the corresponding request form.
Request form for NMR analysis
Request form for mass spectrometry analysis
Rate
A quote is sent after reception of the request form.
Nuclear Magnetic Resonance (NMR)
NMR spectrometer VARIAN Unity-Inova 500 MHz (11.57 Tesla)
4 probes
A 10mm broad band probe (31P-109Ag), a 5mm switchable probe (1H/19F and 31P-109Ag), and a double resonance 3mm inverse probe for
· 10 mm Broad-Band probe (31P - 109Ag)
· 5 mm switchable probe ( 1H/19F and 31P - 109Ag)
· a double resonance 3 mm probe, equipped with a z-axis gradient, for 1H observation and 15N/13C decoupling.
· a double resonance 5 mm probe, equipped with a z-axis gradient, for 1H observation and 15N/13C decoupling
Experiments
· 1D experiments 1H, 19F and X (31P-109Ag) ; 1H /X decoupling; X/1H decoupling
· 1D multi-impulsion experiments (DETP, INEPT, mesure de T1 et de T2)
· Homonoclear 2D experiments 1H-1H : COSY, TOCSY, NOESY, ROESY
· Heteronuclear 2D experiments 1H-X : HMQC, HSQC, HMBC
· Relative and absolute quantification of compounds in mixtures
Mass spectrometry
Structural Characterisation and High Dynamic Range Non-Targeted Analysis (HRMS):
UHPLC - Q-Orbitrap High Resolution Mass Spectrometer (Thermo's Exploris 480)
Complex Structural characterisation (IMS-QTOF):
UHPLC - Trapped Ion Mobility Q-TOF High Resolution Mass Spectrometer (Bruker's timsTOF)
Quantification and high throughput targeted analysis (SRM/MRM):
UHPLC - Triple Quadrupole Mass Spectrometer (Waters' TQD)
Highlights on some developments and achievements:
Rosé Wine polyphenols / Grape Tannins composition / Glutathion and GRP quantitation in wine / Polyphenol polymerization: A New Way to analyze complex HRMS data (Petrolomics based data analysis) / New reactive intermediate moeties participate to tannins complexity / New polyphenols identified in dried-fermented cocoa beans / Prediction of chocolate organoleptic quality based on cocoa bean polyphenol analysis.
Chemometrics
Chemometrics includes a range of data analysis techniques enabling to build stochastic models in order to predict one or more characteristics of the studied samples, starting from their spectra (MS, NMR, UV-visible, IR). Demands regarding such studies, requiring acquisition of a spectral library, must be examined by the platform committee.
Softwares:
SAS (Statistical Analysis System)
Matlab
Scilab (a free mathematics software) with the module FACT (Free Access Chemometric Toolbox) developed and maintained by the polyphenol analysis platform.
Recent publications (2015-...):
Fayeulle N, Meudec E, Boulet JC, Vallverdú-Queralt A, Hue C, Boulanger R, Cheynier V, Sommerer N
Fast Discrimination of Chocolate Quality Based On Average Mass Spectra Fingerprints of Cocoa Polyphenols
Journal of Agricultural and Food Chemistry, 2019, 67, 2723-2731 doi: 10.1021/acs.jafc.8b06456
Saad N, Louvet F, Tarrade S, Meudec E, Grenier K, Landolt C, Ouk TS, Bressollier P
Enzyme‐Assisted Extraction of Bioactive Compounds from Raspberry (Rubus idaeus L.) Pomace
Journal of Food Science, 2019, 84, 1371-1381 doi: 10.1111/1750-3841.14625
Bordiga M, Meudec E, Williams P, Montella R, Travaglia F, Arlorio M, Coïsson JD, Doco T
The impact of distillation process on the chemical composition and potential prebiotic activity of different oligosaccharidic fractions extracted from grape seeds,
Food Chemistry, 2019, 423-430 doi: 10.1016/j.foodchem.2019.01.175
Bontpart T, Ferrero M, Khater F, Marlin T, Vialet S, Vallverdú-Queralt A, Pinasseau L, Ageorges A, Cheynier V, Terrier N
Focus on putative serine carboxypeptidase-like acyltransferases in grapevine
Plant Physiology and Biochemistry, 2018 doi: 10.1016/j.plaphy.2018.07.023
Zerbib M, Mazauric JP, Meudec E, Le Guernevé C, Lepak A, Nidetzky B, Cheynier V, Terrier N, Saucier C
New flavanol O-glycosides in grape and wine
Food Chemistry, 2018 doi: 10.1016/j.foodchem.2018.06.019
Liu PH, Vrigneau C, Salmon T, Hoang DA, Boulet JC, Jégou S, Marchal R
Influence of Grape Berry Maturity on Juice and Base Wine Composition and Foaming Properties of Sparkling Wines from the Champagne Region
Molecules, 2018, 23, 1372 doi: 10.3390/molecules23061372 (Open Access Article)
Roger JM, Boulet JC
A review of orthogonal projections for calibration
Journal of Chemometrics, 2018 doi: 10.1002/cem.3045
Fayeulle N, Vallverdú-Queralt A, Meudec E, Hue C, Boulanger R, Cheynier V, Sommerer N
Characterization of new flavan-3-ol derivatives in fermented cocoa beans
Food Chemistry, 2018 (259), 207-212 doi: 10.1016/j.foodchem.2018.03.133
Carrascón V, Vallverdú-Queralt A, Meudec E, Sommerer N, Fernandez-Zurbano P, Ferreira V
The kinetics of oxygen and SO2 consumption by red wines. What do they tell about oxidation mechanisms and about changes in wine composition?
Food Chemistry, 2018 (241), 206-214 doi: 10.1016/j.foodchem.2017.08.090
Ferreira-Lima N, Vallverdú-Queralt A, Meudec E, Pinasseau L, Verbaere A, Bordignon-Luiz MT, Le Guernevé C, Cheynier V, Sommerer N
Quantification of hydroxycinnamic derivatives in wines by UHPLC-MRM-MS
Analytical and Bioanalytical Chemistry, 2017 doi: 10.1007/s00216-017-0759-y
Pinasseau L, Vallverdú-Queralt A, Verbaere A, Roques M, Meudec E, Le Cunff L, Peros JP, Ageorges A, Sommerer N, Boulet JC, Terrier N, Cheynier V
Cultivar diversity of grape skin polyphenol composition and changes in response to drought investigated by LC-MS based metabolomics
Frontiers in Plant Science, 2017, 8, 1826 doi: 10.3389/fpls.2017.01826
Vallverdú-Queralt A, Meudec E, Eder M, Lamuela-Raventos RM, Sommerer N, Cheynier V
The Hidden Face of Wine Polyphenol Polymerization Highlighted by High-Resolution Mass Spectrometry
ChemistryOpen, 2017 doi: 10.1002/open.201700044 (open access article)
Vallverdú-Queralt A, Meudec E, Eder M, Lamuela-Raventos RM, Sommerer N, Cheynier V
Targeted filtering reduces the complexity of UHPLC-Orbitrap-HRMS data to decipher polyphenol polymerization
Food Chemistry, 2017, 255-263 doi: 10.1016/j.foodchem.2017.01.106
Pinasseau L, Verbaere A, Roques M, Meudec E, Vallverdú-Queralt A, Ollier L, Marlin T, Guiraud JL, Berger G, Bertrand Y, Le Cunff L, Péros JP, Ageorges A, Terrier N, Boulet JC, Cheynier V, Sommerer N
InnoVine WP3: 105 phenolic compound quantification of 2014 and 2015 mature grape berries from a core-collection of 279 irrigated and non-irrigated Vitis vinifera cultivars
Zenodo, 2017 [Data set] doi: 10.5281/zenodo.574857
Boulet JC, Ducasse MA, Cheynier V
Ultraviolet spectroscopy study of wine polyphenols and other major compounds in red wines: relationship between astringency and the concentration of phenolic substances
Australian Journal of Grape and Wine Research, 2017, 23: 193-199 doi: 10.1111/ajgw.12265
Charnier C, Latrille E, Jimenez J, Lemoine M, Boulet JC, Miroux J,Steyer JP
Fast characterization of solid organic waste content with near infrared spectroscopy in anaerobic digestion
Waste Management, 2017, 59: 140-148 doi: 10.1016/j.wasman.2016.10.029
Torres-Aquino M, Becquer A, Le Guernevé C, Louche J, Amenc LK, Staunton S, Quiquampoix H, Plassard C
The host plant Pinus pinaster exerts specific effects on phosphate efflux and polyphosphate metabolism of the ectomycorrhizal fungus Hebeloma cylindrosporum: a radiotracer, cytological staining and 31P NMR spectroscopy study
Plant Cell Environment, 2017, 40: 190-202 doi: 10.1111/pce.12847
Pinasseau L, Verbaere A, Roques M, Meudec E, Vallverdú-Queralt A, Terrier N, Boulet JC, Cheynier V, Sommerer N
A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization
Molecules, 2016, 21(10), 1409 doi: 10.3390/molecules21101409 / download pdf (open access article)
Vallverdú-Queralt A, Biler M, Meudec E, Le Guernevé C, Vernhet A, Mazauric JP, Legras JL, Loonis M, Trouillas P, Cheynier V, Dangles O
p-Hydroxyphenyl-pyranoanthocyanins: An Experimental and Theoretical Investigation of Their Acid—Base Properties and Molecular Interactions
International Journal of Molecular Sciences, 2016, 1842; doi: 10.3390/ijms17111842
Ferreira-Lima N, Vallverdú-Queralt A, Meudec E, Mazauric J-P, Sommerer N, Bordignon-Luiz MT, Cheynier V, Le Guernevé C
Synthesis, Identification, and Structure Elucidation of Adducts Formed by Reactions of Hydroxycinnamic Acids with Glutathione or Cysteinylglycine
Journal of Natural Products, 2016, 2211-2222 doi: 10.1021/acs.jnatprod.6b00279
Boulet JC, Trarieux C, Souquet JM, Ducasse MA, Caillé S, Samson A, Williams P, Doco T, Cheynier V
Models based on ultraviolet spectroscopy, polyphenols, oligosaccharides and polysaccharides for prediction of wine astringency
Food Chemistry, 2016, 190, 357-363 doi: 10.1016/j.foodchem.2015.05.062
Bontpart T, Marlin T, Vialet S, Guiraud JL, Pinasseau L, Meudec E, Sommerer N, Cheynier V, Terrier N
Two shikimate dehydrogenases, VvSDH3 1 and VvSDH4, are involved in gallic acid biosynthesis in grapevine
Journal of Experimental Botany, 2016, 3537-3550 doi: 10.1093/jxb/erw184
Marone A, Carmona-Martínez AA, Sireb Y, Meudec E, Steyer JP, Bernet N, Trably E
Bioelectrochemical treatment of table olive brine processing wastewater for biogas production and phenolic compounds removal
Water Research, 2016, 316-325 doi: 10.1016/j.watres.2016.05.008
Vallverdú-Queralt A, Meudec E, Ferreira-Lima N, Sommerer N, Dangles O, Cheynier V, Le Guernevé C
A comprehensive investigation of guaiacyl-pyranoanthocyanin synthesis by one-/two-dimensional NMR and UPLC-DAD-ESI-MSn
Food Chemistry, 2016, 902-910 doi :10.1016/j.foodchem.2015.12.089
Pineda-Vadillo C, Nau F, Guerin Dubiard C, Cheynier V, Meudec E, Sanz-Buenhombre M, Guadarrama A, Tóth T, Csavajda E, Hingyi H, Karakaya S, Sibakov J, Capozzi F, Bordoni A, Dupont D
In vitro digestion of dairy and egg products enriched with grape extracts: Effect of the food matrix on polyphenol bioaccessibility and antioxidant activity
Food Research International, 2016, 284-292 doi: 10.1016/j.foodres.2016.01.029
Dombre C, Wirth J, Toussaint M, Lixon C, Verbaere A, Sommerer N, Boulet JC, Caille S, Cheynier V, Rigou P, Samson A, Salmon J, Vidal J, Marais S, Gerand Y, Roux P, Lemaistre M, Bobe A, Languet P, Chalier P
Le polyéthylène téréphtalate, un emballage pour le vin ? Partie 2/2 : Évolution au cours du stockage d’un vin rosé conditionné en bouteilles PET avec absorbeur d’oxygène
Revue Française d'Oenologie, 2016, 158: 47-50
Dombre C, Wirth J, Toussaint M, Lixon C, Verbaere A, Sommerer N, Boulet JC, Caille S, Cheynier V, Rigou P, Samson A, Salmon J, Vidal J, Marais S, Gerand Y, Roux P, Lemaistre M, Bobe A, Languet P, Chalier P
Le polyéthylène téréphtalate, un emballage pour le vin ? Partie 1/2 : Propriétés barrière, sécurité sanitaire, analyse du cycle de vie
Revue Française d'Oenologie, 2015, 157: 14-44
Lambert M, Meudec E, Verbaere A, Mazerolles G, Wirth J, Masson G, Cheynier V, Sommerer N
A high-throughput UHPLC-QqQ-MS method for polyphenol profiling in rosé wines
Molecules, 2015, 7890-7914 doi: 10.3390/molecules20057890 / download pdf (open access article)
Vallverdú-Queralt A, Verbaere A, Meudec E, Cheynier V, Sommerer N
Straightforward method to quantify GSH, GSSG, GRP and hydroxycinnamic acids in wines by UPLC-MRM-MS
Journal of Agricultural and Food Chemistry, 2015, 142−149 doi: 10.1021/jf504383g
Doco T, Williams P, Meudec E, Cheynier V, Sommerer N
Complex carbohydrates of red wine: Characterization of the extreme diversity of neutral oligosaccharides by ESI-MS
Journal of Agricultural and Food Chemistry, 2015, 671-682 doi: 10.1021/jf504795g
Koffi EN, Meudec E, Adjé FA, Lozano PR, Lozano YF, Bekro YA
Effect of reverse osmosis concentration coupled with drying processes on polyphenols and antioxidant activity obtained from Tectona grandis leaf aqueous extracts
Journal of Applied Research on Medicinal and Aromatic Plants, 2015, 54-59 doi: 10.1016/j.jarmap.2015.03.001
Pérez-Mañá C, Farré M, Rodríguez-Morató J, Papaseit E, Pujadas M, Fitó M, Robledo P, Covas MI, Cheynier V, Meudec E, Escudier JL, de la Torre R
Moderate consumption of wine, through both its phenolic compounds and alcohol content, promotes hydroxytyrosol endogenous generation in humans. A randomized controlled trial.
Molecular Nutrition & Food Research, 2015, 1213-1316 doi: 10.1002/mnfr.201400842
Cheynier V, Tomas-Barberan FA, Yoshida K
Polyphenols: From Plants to a Variety of Food and Nonfood Uses
Journal of Agricultural and Food Chemistry, 2015, 7589-7594 doi: 10.1021/acs.jafc.5b01173
Martin JC, Maillot M, Mazerolles G and 35 others.
Can we trust untargeted metabolomics? Results of the metabo-ring initiative, a large-scale, multi-instrument inter-laboratory study.
Metabolomics, 2015, 807-821 doi: 10.1007/s11306-014-0740-0
Highlight on previous works:
Cheynier V
Phenolic compounds: from plants to foods
Phytochemical Reviews, 2012, 153-177 doi: 10.1007/s11101-012-9242-8
Tarascou I, Souquet JM, Mazauric JP, Carrillo S, Coq S, Canon F, Fulcrand H, Cheynier V
The hidden face of food phenolic composition
Archives of Biochemistry and Biophysics, 2010, 16–22 doi: 10.1016/j.abb.2010.03.018
Mazerolles G, Preys S, Bouchut C, Meudec E, Fulcrand H, Souquet JM, Cheynier V
Combination of several mass spectrometry ionization modes: A multiblock analysis for a rapid characterization of the red wine polyphenolic composition
Analytica Chimica Acta, 2010, 195-202 doi: 10.1016/j.aca.2010.07.034
Coq S, Souquet JM, Meudec E, Cheynier V, Hättenschwiler S
Interspecific variation in leaf litter tannins drives decomposition in a tropical rain forest of French Guiana
Ecology, 2010, 2080-2091 doi: 10.1890/09-1076.1
Fulcrand H, Mané C, Preys S, Mazerolles G, Bouchut C, Mazauric JP, Souquet JM, Meudec E, Li Y, Cole R, and Cheynier V
Direct mass spectrometry approaches to characterize polyphenol composition of complex samples
Phytochemistry, 2008, 3131-3138 doi: 10.1016/j.phytochem.2008.03.016
Mané C, Sommerer N, Yalcin T, Cheynier V, Cole RB, Fulcrand H
Assessment of the molecular weight distribution of tannin fractions through MALDI-TOF MS analysis of protein-tannin complexes
Analytical Chemistry, 2007, 2239-2248 doi: 10.1021/ac061685+
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