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Dernière mise à jour : Mai 2018

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Post-doc Alexandre Chamas

In vivo determination of enzymatic parameters in a metabolic pathway of S. cerevisiae yeast
Alexandrebis

My project is a part of the programm ANR Enzinvivo (2017-2019, leaders: Virginie Galeote, Carole Camarasa, Sylvie Dequin and Thibault Nidelet).

Enzymatic reactions are key components of yeast metabolic pathways and synthetic biology is all about modifying these reactions to obtain a desired product. In my current project, I try to decipher how enzymatic reactions work inside a yeast cell and to determine the ideal concentrations of intermediate metabolites in order to maximize the production of a target molecule. Therefore the first part of my work consist in creating several modified yeast strains by molecular biology techniques (genome editing by CRISPR-Cas9, promoter modifications) and then analyse how these modifications impact a designated metabolic pathway by performing transcriptomics, proteomics and metabolomics as well as flux balance analysis.

Contact : Alexandre Chamas (alexandre.chamas@inra.fr)

More information :

https://www.linkedin.com/in/alexandre-chamas-92979684/

https://www.researchgate.net/profile/Alexandre_Chamas

Main publications :

Chamas A., Pham H. T. M., Jahne M., Hettwer K., Uhlig S., Simon K., Einspanier A., Baronian K. and Kunze G. (2017) Simultaneous detection of three sex steroid hormone classes using a novel yeast-based biosensor. Biotechnol Bioeng, 7: 1539-49

Chamas A., Pham H. T. M., Jahne M., Hettwer K., Gehrmann L., Tuerk J., Uhlig S., Simon K., Baronian K. and Kunze G. (2017) Separation and identification of hormone-active compounds using a combination of chromatographic separation and yeast-based reporter assay. Sci Total Environ, 605-606: 507-513

Chamas A., Pham H.T.M., Baronian K., Kunze G. (2017) Biosensors Based on Yeast/Fungal Cells. In: Sibirny A. (eds) Biotechnology of Yeasts and Filamentous Fungi. Springer, Cham. DOI : https://doi.org/10.1007/978-3-319-58829-2_12

Bischoff F., Chamas A., Litwińska K., Matthes F., Böer E., Kunze G. (2017) Applications of Blastobotrys (Arxula) adeninivorans in Biotechnology. In: Satyanarayana T., Kunze G. (eds) Yeast Diversity in Human Welfare. Springer, Singapore. DOI : https://doi.org/10.1007/978-981-10-2621-8_18

Chamas A., Giersberg M., Friedrich K., Sonntag F., Kunze D., Uhlig S., Simon K., Baronian K. and Kunze G. (2015) Purification and immunodetection of the complete recombinant HER-2[neu] receptor produced in yeast. Protein Expr Purif, 105: 61-70

Chamas A., Nieter A., Pham H.T.M., Giersberg M., Hettwer K., Uhlig S., Simon K., Baronian K. and Kunze G. (2015) Development of a recombinant Arxula adeninivorans cell bioassay for the detection of molecules with progesterone activity in wastewater. Anal Bioanal Chem, 407: 8109-20